The advent of single-cell, high-dimensional imaging technologies have enabled unprecedented analysis of solid tumors without loss of structural and architectural features. We are using MIBI-ToF to analyze primary neuroblastoma tumors. Neuroblastoma is the most common extracranial solid tumor of childhood and has diverse outcomes based on known clinical features. We are studying neuroblastoma at the single-cell level to better understand the immune landscape of neuroblastoma tumors and how tumor cells of different lineages (mesenchymal vs. adrenergic) co-exist within the tumor ecosystem.